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Telomerase inhibitors based on quadruplex ligands selected by a fluorescence assay

โœ Scribed by Mergny, J.-L.; Lacroix, L.; Teulade-Fichou, M.-P.; Hounsou, C.; Guittat, L.; Hoarau, M.; Arimondo, P. B.; Vigneron, J.-P.; Lehn, J.-M.; Riou, J.-F.; Garestier, T.; Helene, C.

Book ID
124087980
Publisher
National Academy of Sciences
Year
2001
Tongue
English
Weight
129 KB
Volume
98
Category
Article
ISSN
0027-8424

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A high-throughput, nonradioactive telomerase assay using a europium-labeled oligonucleotide probe and time-resolved fluorescence has been developed to detect PCR-amplified telomerase products. The use of a thermally activated TAQ polymerase, rather than a wax barrier, to implement a PCR hot-start fa