Television microscopy of sporulation and spore germination of Bacillus cereus

✍ Scribed by Zworykin, Katherine A. ;Chapman, George B.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1956
• Tongue: English
• Weight: 760 KB
• Volume: 48
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030480211

✦ Synopsis

THIRTEEN FIQURES

An extensive review of the literature has revealed only a few papers which present the results of observations of living sporulating and germinating bacteria. Of these papers, the most informative have been those by Wyckoff and Ter Louw ( '31), who used ultraviolet microscopy, Bayne-Jones and Petrilli ( '33), who used cinematography and Knaysi ( '46, '52), who employed light and phase microscopy.

A new method, that of television microscopy, facilitates continuous observation of living cells, and provides a means for controlling the optical contrast of cells and their components, (Parpart, '51; Flory, '51; Zworykin and Flory, '52).

The present study has found this method useful in determining the fate of certain cytoplasmic inclusions. I n addition, some new observations on the processes of sporulation and germination are described.

MSTERIALS AND METHODS

The culture used in this work was the A.T.C.C. strain no.

7064 of BaciZZzcs cereus. Following 24 hours of growth at 30°C. in nutrient broth, a 3mm loop of the culture was transferred to the surface of a nutrient agar slant and incubated at 30°C. for 24 hours.