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Techniques to investigate mitochondrial function in neurons

✍ Scribed by Strack, Stefan;Usachev, Yuriy M


Publisher
Humana Press
Year
2017
Tongue
English
Leaves
350
Series
Spriner protocols; Neuromethods 123
Category
Library

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✦ Table of Contents


Neuromethods Preface...Preface... Table of Contents... Contributing Authors... 1. Three-Dimensional Reconstruction of Neuronal Mitochondria by Electron Tomography Guy Perkins 2. Measuring Mitochondrial Shape with ImageJ Ronald A. Merrill, Kyle H. Flippo, and Stefan Strack 3. Live Imaging Mitochondrial Transport in Neurons Meredith M. Course, Chung-Han Hsieh, Pei-I Tsai, Jennifer A. Codding-Bui, Atossa Shaltouki, and Xinnan Wang 4. Techniques to Investigate Bioenergetics of Mitochondria William I. Sivitz 5. Respirometry in Neurons Liang Zhang and Eugenia Trushina 6. Measuring ATP in Axons with FRET Lauren Y. Shields, Bryce A. Mendelsohn, and Ken Nakamura 7. Characterizing Metabolic States using Fluorescence Lifetime Imaging Microscopy (FLIM) of NAD(P)H Thomas S. Blacker and Michael R. Duchen8. Techniques for Simultaneous Mitochondrial and Cytosolic Ca2+ Imaging in NeuronsJacob E. Rysted, Zhihong Lin, and Yuriy M. Usachev 9. Live Imaging of Mitochondrial ROS Production and Dynamic Redox Balance in Neurons Karolina Can, Sebastian Kugler, and Michael Muller 10. Monitoring of Permeability Transition Pore Openings in Isolated Individual Brain Mitochondria Nickolay Brustovetsky and Tatiana Brustovetsky 11. Examination of Mitochondrial Ion Conductance by Patch Clamp in Intact Neurons and Mitochondrial Membrane Preparations Elizabeth A. Jonas and Nelli Mnatsakanyan 12. Monitoring of Permeability Transition Pore Openings in Mitochondria of Cultured Neurons Tatiana Brustovetsky and Nickolay Brustovetsky 13. Protocols for Assessing Mitophagy in Neuronal Cell Lines and Primary Neurons Ruben K. Dagda and Monica Rice 14. Examining Mitochondrial Function at Synapses In Situ Gregory T. Macleod and Maxim V. Ivannikov 15. Proteomic Analysis of Neuronal Mitochondria Kelly L. Stauch and Howard S. Fox 16. Measuring Mitochondrial Pyruvate Oxidation Lawrence R. Gray, Alix A.J. Rouault, Lalita Oonthonpan, Adam J. Rauckhorst, Julien A. Sebag, and Eric B. Taylor

✦ Subjects


Mitochondrium;Nervenzelle


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