<p>The Sample Preparation Techniques for Environmental, Plant, and Animal Samples handbook is a collection of best practices, recipes and theoretical information aimed at anyone who works with any type of molecular biology, proteomics, or metabolomics research involving diffi cult and tough-to-proce
Techniques for Work with Plant and Soil Nematodes
โ Scribed by Roland N. Perry (editor), David Hunt (editor), Sergei Subbotin (editor)
- Publisher
- CABI
- Year
- 2020
- Tongue
- English
- Leaves
- 313
- Category
- Library
No coin nor oath required. For personal study only.
โฆ Synopsis
Techniques for Work with Plant and Soil Nematodes is an up-to-date, comprehensive book covering the practicalities of working with and studying soil and plant nematodes. Written by an international team of experts, this book is highly illustrated and provides thorough coverage of methods while allowing for relevant information to be located quickly. It includes the fundamental traditional techniques and new methodologies, covering: sampling; extraction; estimating numbers; handling, fixing, staining, mounting; culturing techniques; figure preparation, measurement and image processing; electron microscopy techniques; behavioral and physiological assays; and cytogenetic, biochemical and molecular biology techniques. This book is an essential resource for anyone involved in plant nematology needing to refer to a readily available methodology standard, including students of nematology and parasitology, university lecturers and researchers, diagnostic laboratories, and quarantine and advisory service personnel. It provides a much needed compendium of the spectrum of information needed to work with these microscopic organisms.
โฆ Table of Contents
Contents
About the Editors
Contributors
Preface
1 Sampling โข Loes J.M.F. den Nijs, Jon Pickup and Ralf-Udo Ehlers
2 Methods for Nematode Extraction โข Nicole Viaene, Johannes Hallmann and Leendert P.G. Molendijk
3 Estimating Numbers โข Johannes Hallmann, Matthias Daub and Wim Wesemael
4 Screening Plants for Resistance/Susceptibility to Plant-parasitic Nematodes โข Wim Wesemael
5 Handling, Fixing, Staining and Mounting Nematodes โข Jonathan D. Eisenback and David J. Hunt
6 Culturing Techniques โข Rosa H. Manzanilla-Lรณpez and Ralf-Udo Ehlers
7 Measuring Nematodes and Preparation of Figures โข Weimin Ye and David J. Hunt
8 Electron Microscopy Techniques โข Vladimir V. Yushin, Myriam Claeys, Jan L.M. Leunissen and Julia K. Zograf
9 Behavioural and Physiological Assays โข Roland N. Perry
10 Staining Chromosomes โข Jonathan D. Eisenback
11 Isoelectric Focusing of Proteins โข Janete A. Brito
12 Molecular Identification of Nematodes using Polymerase Chain Reaction (PCR) โข Sergei A. Subbotin
13 Isolation and Characterization of Tandem Repeats in Nematode Genomes โข Philippe Castagnone-Sereno
14 Characterization of Nematode Mitochondrial Genomes โข Danny A. Humphreys-Pereira, Taeho Kim and Joong-Ki Park
15 Phylogenetic Analysis of DNA Sequence Data โข Sergei A. Subbotin
Index
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