Technicon Immuno 1® PSA assay measures both free and alpha-1-antichymotrypsin-complexed prostate-specific antigen on an equimolar basis
✍ Scribed by Zeqi Zhou; Phillip C. Ng; Donald L. Very Jr.; W. Jeffrey Allard; Kwok K. Yeung
- Book ID
- 102657234
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 549 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0887-8013
No coin nor oath required. For personal study only.
✦ Synopsis
In this study, we investigated the immunoreactivity of theTechnicon lmmuno I'PSA assay, a monoclonal-polyclonal sandwich immunoassay, with free and ACT-complexed PSA. Assay calibrators prepared with free PSA (standard lmmuno 1 calibrators) and calibrators consisting of 9O%ACT-complexed and 10% free PSA (9O:lO calibrators) yielded virtually identical PSA recoveries at all concentrations tested. Concentrations of total PSA at -4 and 10 ng/mL, prepared in varying ratios of free PSA to PSA-ACT complex, recovered from 92-104% at 4 ng/mL and from 98-1 02% at 10 ng/mL. Additionally, an excellent correlation of serum total PSA values from a panel of 40 prostatic cancer patient samples was obtained using calibration curves generated with the standard lmrnuno 1 calibrators or the 9O:lO calibrators. These results demonstrate that the Technicon lmmuno I @ PSA assay measures free and ACT-complexed PSA on an equal molar basis.