Technical and statistical improvements for flow cytometric DNA analysis of paraffin-embedded tissue
โ Scribed by Dr. Brenda J. Sickle-Santanello; William B. Farrar; Jane F. Decenzo; Sedigheh Keyhani-Rofagha; John Klein; Dennis Pearl; Harry Laufman; Robert V. O'Toole
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 757 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
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## Abstract It has recently been shown that bimodal histograms with false aneuploid peaks may be obtained by DNA flow cytometry from histologically normal tissue allowed to autolyze. To investigate if such peaks can be generated from surgically excised archival tissue, 198 paraffin blocks from 179
A modification of the Hedley-method for flow cytometric DNA analysis of paraffin-embedded tissues is presented. Dewaxed and dehydrated tissue from paraffin blocks was incubated with subtilisin Carlsberg (pronase, Sigma protease XXIV) and then stained directly without washing and centrifugation. The
We have developed a simple, rapid method for isolating cells from a block of formalin-fixed, paraffin-embedded tissue specimen for laser scanning cytometric (LSC) DNA analysis by using a grater. The scraping-like tissue samples were obtained by grating a paraffin-embedded tissue block. The grated sa