๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Technical and statistical improvements for flow cytometric DNA analysis of paraffin-embedded tissue

โœ Scribed by Dr. Brenda J. Sickle-Santanello; William B. Farrar; Jane F. Decenzo; Sedigheh Keyhani-Rofagha; John Klein; Dennis Pearl; Harry Laufman; Robert V. O'Toole


Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
757 KB
Volume
9
Category
Article
ISSN
0196-4763

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


DNA flow cytometry of paraffin-embedded
โœ David W. Hedley; Michael L. Friedlander; Ian W. Taylor; Catherine A. Rugg; Eliza ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 94 KB ๐Ÿ‘ 1 views
Evidence for false aneuploid peaks in fl
โœ Heikki Joensuu; Kalle A. Alanen; Pekka J. Klemi; Risto Aine ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 474 KB ๐Ÿ‘ 2 views

## Abstract It has recently been shown that bimodal histograms with false aneuploid peaks may be obtained by DNA flow cytometry from histologically normal tissue allowed to autolyze. To investigate if such peaks can be generated from surgically excised archival tissue, 198 paraffin blocks from 179

An improved hedley method for preparatio
โœ Thomas Heiden; Naining Wang; Dr. Bernhard Tribukait ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 616 KB

A modification of the Hedley-method for flow cytometric DNA analysis of paraffin-embedded tissues is presented. Dewaxed and dehydrated tissue from paraffin blocks was incubated with subtilisin Carlsberg (pronase, Sigma protease XXIV) and then stained directly without washing and centrifugation. The

Sample preparation from paraffin-embedde
โœ Tomomi Kamada; Kohsuke Sasaki; Tatsuo Tsuji; Takeshi Todoroki; Masahiro Takahash ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 220 KB

We have developed a simple, rapid method for isolating cells from a block of formalin-fixed, paraffin-embedded tissue specimen for laser scanning cytometric (LSC) DNA analysis by using a grater. The scraping-like tissue samples were obtained by grating a paraffin-embedded tissue block. The grated sa