Taxonomic implication of the apparent undetectability of 3-deoxy-pD-manno-2-octulosonate (Kdo) in lipopolysaccharides of representatives of the family vibrionaceae and the occurrence of Kdo 4-phosphate in their inner-core regions
✍ Scribed by Seiichi Kondo; Yuju Haishima; Kazuhito Hisatsune
- Book ID
- 102998694
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 680 KB
- Volume
- 231
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
After conventional hydrolysis of lipopolysaccharides (LPSs), Kdo was not detectable by the periodate-thiobarbituric acid test in those of any member of Vibrionaceae except the gems Plesiomonas, but phosphorylated Kdo was demonstrated after strong-acid hydrolysis. Dephosphorylation, periodate oxidation, and methylation analysis of LPS preparations from 7 strains selected from all genera of Vibrionaceae, except Plesiomonas, showed that the inner-core region (unlike that in enteric Gram-negative bacteria) contains only one molecule of Kdo 4-phosphate 5-substituted with heptose, a constituent of the distal part of the core region, as in enteric bacteria. The undetectability of Kdo in LPS after conventional hydrolysis and the occurrence of phosphorylated Kdo in strong-acid hydrolysates and of Kdo 4-phosphate in the inner-core region are taxonomic characteristics of the family Vibrionaceae.