Taurine Release Is Enhanced in Cell-Damaging Conditions in Cultured Cerebral Cortical Astrocytes
β Scribed by Pirjo Saransaari; Simo S. Oja
- Book ID
- 110405170
- Publisher
- Springer
- Year
- 1999
- Tongue
- English
- Weight
- 667 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0364-3190
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## Abstract Hyposmolarity (β30%) in cultured cerebellar astrocytes raised cytosolic Ca^2+^ concentration ([Ca^2+^]~i~) from 160 to 400 nM and activated the osmosensitive taurine release (OTR) pathway. Although OTR is essentially [Ca^2+^]~i~βindependent, further increase in [Ca^2+^]~i~ by ionomycin
The properties of [3H]glycine uptake and release were studied with cerebellar granule cells, 7-9 days in vitro, (DIV) and astrocytes, 14-15 DIV, in primary cultures. The uptake of glycine in both cell types consisted of a saturable high-affinity transport and nonsaturable diffusion. The transport co