Targeting of a foreign protein into the thylakoid lumen of pea chloroplasts
β Scribed by Julie W. Meadows; Jamie B. Shackleton; Andrew Hulford; Colin Robinson
- Book ID
- 115922121
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 228 KB
- Volume
- 253
- Category
- Article
- ISSN
- 0014-5793
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A full-length cDNA clone encoding the PSI-F subunit of barley photosystem I has been isolated and sequenced. The open reading frame encodes a precursor polypeptide with a deduced molecular mass of 24 837 Da. The barley PSI-F precursor contains a bipartite presequence with characteristics similar to
A 64-kilodalton (kDa) protein, situated in the lumen between the inner and outer envelopes of pea (Pisum sativum L.) chloroplasts (Soll and Bennett 1988, Eur. J. Biochem., 175, 301-307) is shown to undergo reversible phosphorylation in isolated mixed envelope vesicles. It is the most conspicuously l