Targeting microspheres and cells to polyethylene glycol-modified biological surfaces

✍ Scribed by Timothy E. Deglau; Jermaine D. Johnson; Flordeliza S. Villanueva; William R. Wagner

• Book ID: 102294755
• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 230 KB
• Volume: 81A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.31092

✦ Synopsis

Abstract

It has previously been demonstrated that damaged arterial tissue can be acutely modified with protein‐reactive polyethylene glycol (PEG) to block undesirable platelet deposition. This concept might be expanded by employing PEG‐biotin and its strong interaction with avidin for site‐specific targeted delivery. Toward this end, cultured endothelial cells (ECs) were surface modified with PEG‐biotin and the available biotin was quantified with flow cytometry. NeutrAvidin‐coated microspheres and PEG‐biotin modified ECs with NeutrAvidin as a bridging molecule were delivered under arterial shear stress to PEG‐biotin modified ECs on a coverslip as well as scrape‐damaged bovine carotid arteries. After incubation with a 10 m__M__ solution for 1 min, 8 × 10^7^ PEG‐biotin molecules/EC were found and persisted for up to 120 h. Perfused microspheres adhered to NHS‐PEG‐biotin treated bovine carotid arteries with 60 ± 16 microspheres/mm^2^ versus 11 ± 4 microspheres/mm^2^ for control arteries (p < 0.015). Similarly, 22 ± 5 targeted ECs/mm^2^ adhered to NHS‐PEG‐biotin treated bovine carotid arteries versus 6 ± 2 ECs/mm^2^ for control arteries (p < 0.01). The targeting strategy demonstrated here might ultimately find application for drug delivery, gene therapy, or cell therapy where localization to specific labeled vascular regions is desired following catheter‐based or surgical procedures. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res 2006