Targeted insertion of an IRES Cre into the Hnf4α locus: Cre-mediated recombination in the liver, kidney, and gut epithelium
✍ Scribed by Stéphane D. Vincent; Elizabeth J. Robertson
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 475 KB
- Volume
- 39
- Category
- Article
- ISSN
- 1526-954X
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✦ Synopsis
Abstract
Summary: The __Hnf4__α gene belongs to a family of trancriptional regulators required for liver development and function. __Hnf4__α is also expressed in other tissues, including the newly formed visceral endoderm of the early postimplantation embryo, and later in embryogenesis in the gut epithelium and the kidney. The regulatory sequences involved in controlling expression of __Hnf4__α at these diverse sites are not clearly understood. Here we used homologous recombination to introduce Cre recombinase coding sequences into the endogenous __Hnf4__α locus. Crossing __Hnf4__α^Creex2/+^ mice with R26^R^ partners allowed us to follow the pattern of Cre‐mediated recombination. Our results show that recombination of the reporter allele closely follows endogenous __Hnf4__α expression, but with a slight temporal delay. Thus, the __Hnf4__α^Creex2^ strain should prove useful for conditionally deleting gene activity in the liver, gut epithelium, or kidney. genesis 39:206–211, 2004. © 2004 Wiley‐Liss, Inc.