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Targeted gene conversion in a mammalian CD34+-enriched cell population using a chimeric RNA/DNA oligonucleotide

โœ Scribed by Yufei Xiang; Allyson Cole-Strauss; Kyonggeun Yoon; Jeffrey Gryn; Eric B. Kmiec


Book ID
105797349
Publisher
Springer
Year
1997
Tongue
English
Weight
699 KB
Volume
75
Category
Article
ISSN
0946-2716

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Targeted nucleotide exchange in the alka
โœ B T Kren; A Cole-Strauss; E B Kmiec; C J Steer ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 597 KB

Although a variety of methods has been devised for modification of hepatic genes, none has been effective for long-term correction of genetic disorders. In this study, we employed a recently described novel experimental strategy for site-directed nucleotide exchange in genomic DNA of HuH-7 human hep