Tandemly Repeated 147 bp Elements Cause Structural and Functional Variation in DivergentMAL Promoters ofSaccharomyces cerevisiae
✍ Scribed by Bell, Philip J. L.; Higgins, Vincent J.; Dawes, Ian W.; Bissinger, Peter H.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 156 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0749-503X
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✦ Synopsis
We have studied four novel MAL promoters isolated from a single strain of bakers' yeast. Within these promoters we have identified up to five tandem 147 bp repeats located between the MAL UAS region and the MALT TATA box. These repeats strongly reduce MALT (maltose permease) gene expression but only weakly reduce MALS (maltase) gene expression. Insertion of the 147 bp elements into the heterologous CYC1 promoter reduced expression when located between the CYC1 UAS and the TATA box, but not when located upstream of the UAS. We propose that these naturally occurring repeats have evolved as a mechanism to lower the level of MALT expression relative to MALS expression, thus avoiding possible toxic effects associated with over-expression from multiple copies of the permease gene.