Tandem mass spectrometry study ofC-phenyl-N-tert-butyl nitrone spin adducts from in vitro rat liver microsomal metabolism of bromotrichloromethane and carbon tetrachloride
✍ Scribed by Edward G. Janzen; Hong Sang; Yashige Kotake; Coit M. DuBose; J. Lee Poyer; Masana Arimura
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 669 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1044-0305
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✦ Synopsis
Electron ionization and thermospray were used in conjunction with tandem mass spectrometry methods to identify trichloromethyl/C-phenyl-N-tert-butyl nitrone (PBN) spin adducts produced in rat liver microsomal dispersions that had been treated with reduced nicotinamide adenine dinucleotide phosphate (NADPH)-generating system and BrCC13 (or CC14).
In the identification of PBN spin adducts, a scan of precursors of m/z 57 was utilized to confirm the presence of PBN spin adducts, because PBN spin adducts produce m/z 57 from tert-butyl as a characteristic fragment. Use of deuterated PBN (PBN-d 9 deuterated at tertbutyl; PBN-d14 deuterated at both phenyl and tert-butyl) improved the recognition of PBN adducts in mixtures by precursor ion scans, because m/z 66 (which corresponds to the deuterated tert-butyl group) is characteristic and, unlike m/z 57, it is not a common fragment for any other compounds. Two new PBN spin adducts that were not detected before by electron paramagnetic resonance or mass spectrometry were identified by these methods for the first time. (]