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Tandem mass spectrometric identification of eicosanoids: Leukotrienes and hydroxyeicosatetraenoic acids

✍ Scribed by Leesa J. Deterding; John F. Curtis; Kenneth B. Tomer

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
811 KB
Volume
21
Category
Article
ISSN
1076-5174

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✦ Synopsis

The product ion spectra of the fast atom bombardment (FAB) desorbed [ M -HIions of leukotrienes (LTs) and hydroxyeicosatetraenoic acids (HETEs) have been obtained on a tandem magnetic sector mass spectrometer with introduction by coaxial continuous flow FAB. Upon collisional activation, the product ion spectra of the [ M -HIions of these derivatives of arachidonic acid reveal structurally informative product ions. The location of the hydroxy groups on the fatty acid chain, and the position and type of the cysteine-containing moiety of the LTs, can be readily determined from the product ion spectra. Tandem mass spectrometry was used to identify 11and 15-HETE produced in a low-temperature microsomal enzymatic oxidation of arachidonic acid.

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