T4 Bacteriophage Gene 32: A Structural Protein in the Replication and Recombination of DNA
β Scribed by ALBERTS, BRUCE M.; FREY, LINDA
- Book ID
- 109674204
- Publisher
- Nature Publishing Group
- Year
- 1970
- Tongue
- English
- Weight
- 716 KB
- Volume
- 227
- Category
- Article
- ISSN
- 0028-0836
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From +29-infected Bacillus subtilis cells, we have isolated a protein fraction which promotes in vitro replication of +29 DNA. This fraction catalyses both initiation and elongation, indicating that it contains the product of gene 3 (tp: terminal protein) and the product of gene 2 (gp2: probably a D
A restriction fragment of lambdaDNA carrying the P gene was cloned in the high copy number plasmid RSF2124. Cells harbouring this new plasmid RSF2124/lambdaE complement lambdaPam80 phage. A lac promoter-operator region (lacP), produced by EcoRI digestion of plasmid pKB252, was inserted into RSF2124/