T4 and ultrasensitive TSH immunoassays using luminescent enhanced xanthine oxidase assay
✍ Scribed by Baret, A. ;Fert, V.
- Publisher
- John Wiley and Sons
- Year
- 1989
- Weight
- 267 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
The use of xanthine oxidase in immunoanalysis has never been reported. We describe here a procedure in which the xanthine oxidase dependent luminescence of luminol is enhanced in the presence of FeEDTA complex, providing an highly sensitive assay (3 amol of enzyme) and a long-term signal.
This specific amplification has been applied t o T4 and ultrasensitive TSH solid phase immunoassays, with T4-X0 and anti-TSH monoclonal antibody-XO conjugates as tracers. The performances of these assays are at least equivalent t o those obtained with iodinated tracers, using the same solid phases and the same calibrators. The major advantages of these immunoassays are: (1) the long-term signal which can be repeatedly recorded over several days, (2) the high detection sensitivity, (3) the long-term stability of the luminescence reagent and (4) the stability of the conjugates.