T3 × T7 phage crosses leading to recombinant RNA polymerases
✍ Scribed by BEIER, HILDBURG; HAUSMANN, RUDOLF
- Book ID
- 109693044
- Publisher
- Nature Publishing Group
- Year
- 1974
- Tongue
- English
- Weight
- 345 KB
- Volume
- 251
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/251538a0
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We determined the nucleotide sequence of gene 1 of Klebsiella phage K11, which is a member of the T7 group of phages. The largest open reading frame corresponds to a polypeptide with 906 amino acids and a molecular weight of 100,383 daltons. The deduced amino acid sequence of this polypeptide shows
## Abstract Double‐stranded DNA can be readily adsorbed on mica or directly on carbon coated grids from the surface of solutions containing ethidium bromide, actinomine, or propidium diiodide. The DNA molecules are unfolded, well separated, and show a length distribution similar to molecules prepar