T cell receptor-γδ-expressing fetal mouse thymocytes are generated without T cell receptor Vβ selection

✍ Scribed by Elisabeth Mertsching; Rhodri Ceredig

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 741 KB
• Volume: 26
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830260412

✦ Synopsis

Vp selection

We investigated whether fetal mouse T cell receptor (TCR) y6 cells have been subjected to so-called TCRP selection at the CD25 stage of thymus development. To this end, we carried out a comparative three-color flow microfluorimetric analysis of TCRyG cells developing in the fetal, neonatal and adult thymus using monoclonal antibodies to CD2, CD8, CD24, CD25 and CD44. Day-15 fetal TCRy6 cells were CD2', suggesting an origin at a post-CD25 stage. Molecular analysis of TCRP rearrangements were also carried out. Thus, by semi-quantitative polymerase chain reaction (PCR) amplification of VP6 and VP8 to JP2 rearrangements day-15 fetal TCRyi3 showed extensive TCRP rearrangements, a finding confirmed by PCR amplification from single micromanipulated cells. Finally, sequencing analysis of 104 PCR-amplified TCR VDJP2 fragments showed that the majority (58 YO) were rearranged out of frame. Taken together, these phenotypic and molecular analyses suggest that fetal TCRyG cells have not been subject to TCRP selection.