T cell receptor β-chain third complementarity-determining region gene usage is highly restricted among Sm-B autoantigen–specific human T cell clones derived from patients with connective tissue disease
✍ Scribed by Beth L. Talken; Margaret-Mary Holyst; David R. Lee; Robert W. Hoffman
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 155 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0004-3591
No coin nor oath required. For personal study only.
✦ Synopsis
Objective:
To determine the structure of t cell receptors (tcr) used by sm-b-reactive human t cell clones, to map t cell epitopes on the sm-b autoantigen, and to determine the hla restriction element used in the recognition of sm-b by t cells.
Methods:
Sm-b-reactive t cell clones were generated from patients with connective tissue disease by using either a recombinant fusion protein or synthetic peptides. the tcr structure was defined with the use of polymerase chain reaction and dna sequencing. synthetic peptides were used to map t cell epitopes on sm-b. hla restriction element usage was defined by using monoclonal antibody blocking.
Results:
Usage of the tcr third complementarity-determining region (cdr3) was highly restricted among sm-b autoantigen-specific human t cell clones. only amino acids 48-96 of the sm-b2 autoantigen were recognized by t cells, and this occurred in the context of hla-dr.
Conclusion:
Tcr cdr3 gene usage is highly conserved by sm-b autoantigen-specific t cell clones, and this appears to be related to the recognition of a limited number of t cell epitopes on the sm-b autoantigen presented in the context of hla-dr.