T cell receptor β chain gene rearrangements and Vβ gene usage in horse cytochrome c-specific T cell hybridomas

✍ Scribed by Susanne Baumhüter; Nicolas Fasel; Giampietro Corradin; John Guardiola

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 576 KB
• Volume: 17
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830170512

✦ Synopsis

T cell receptor p chain gene rearrangements and V, gene usage in horse cytochrome c-specific T cell hybridomas*

Comparison of T cell receptor a and $-chain genes in murine major histocompatibility complex (MHC) class I and class 11-restricted T cell clones and hybridomas recognizing different antigens indicates that no simple correlation exists between the observed antigen/MHC specificity and the expression of certain a and P-chain heterodimers.

We have attempted to establish a possible correlation by analyzing T cell receptor (3 chain gene rearrangements and Vp gene usage in five T cell hybridomas with identical antigen/MHC specificity and another hybridoma recognizing a different antigenic determinant in association with the same restriction molecule. We report here that in each of the five clones a uniquely rearranged $ chain gene is expressed in combination with at least two different Vp gene segments. The presence of the differently rearranged T cell receptor P chain genes correlated with the finding of distinct fine specificity pattern of antigen recognition in each of the hybridomas. Interestingly, two hybridomas specific for different epitopes showed identical $ chain D-J rearrangements indicating that the differences might be encoded by the a chain gene orland the V, gene element.