T-cell derived cytokines co-stimulate proliferation of CD40-activated germinal centre as well as follicular lymphoma cells
✍ Scribed by Doris Schmitter; Michael Koss; Eva Niederer; Rolf Arno Stahel; Gabriella Pichert
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 236 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0278-0232
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✦ Synopsis
Follicular lymphomas, the malignant counterparts of normal germinal centre (GC) B-cells, grow in vivo in close association with polyclonal T-cells, predominantly from the T-helper cell type. T-cell-derived growth factors are involved in the development of GC B-cells. However, their role in the pathogenesis of follicular lymphomas has not been clearly defined. We investigated the co-stimulatory activity of 14 cytokines (interleukin-1 to -8, IL-10, IL-13, IFN-, TNF-, GM-CSF and SCF) on the proliferation of CD40activated follicular lymphoma cells in comparison to tonsillar GC B-cells. Tonsillar GC B-cells (n=4), malignant cells from diagnostic lymph node biopsies of patients with follicular (n=4) or transformed (n=4) lymphomas were grown on irradiated CD40-ligand transfectants, with and without cytokines.
[ 3 H]-thymidine uptake was measured at day 7. IL-10 and IL-4 proved to be the most potent co-stimulators of proliferation of tonsillar GC B-cells, whereas proliferation of follicular lymphoma cells was co-stimulated by IL-4. The fact that IL-4 is a T-cell derived cytokine, suggests that lymphoma infiltrating T-cells play a role in the growth of these malignancies. Moreover, proliferation of both non-neoplastic tonsillar GC B-cells and follicular lymphomas is co-stimulated by T-cell derived cytokines, indicating that responsiveness to paracrine factors may not be a characteristic of the malignant phenotype. 1997 John Wiley & Sons, Ltd.