T cell activation: independent induction of killing activity and interleukin 2 secretion in cytolytic hybridomas*

✍ Scribed by Tali Ozery; Gideon Berke; Miriam Moscovich; Keiko Ozato; Yael Kaufmann

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 398 KB
• Volume: 19
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830191032

✦ Synopsis

T cell activation: independent induction of killing activity and interleukin 2 secretion in cytolytic hybridomas"

Memory-like cytotoxic T lymphocytes (CTL) hybridomas exhibiting inducible killing activity and IL2 production were used to analyze the anamnestic response of CTL. Four activating agents were examined; anti-Thy-1 monoclonal antibody G7, staphylococcal enterotoxin B, interferon (IFN)-a/B and IFN-y. These agents seemed to affect CTL activities in three distinct ways. Anti-Thy-1 monoclonal antibody, like specific antigen, was found to be a potent inducer of specific killing and IL2 production, whereas staphylococcal enterotoxin B induced IL 2 production, but not cytolytic activity. On the other hand, IFN-cdg and IFN-y effectively stimulated cytotoxicity without inducing IL 2 production. The independent triggering of specific killing and IL 2 secretion in the monoclonal cytolytic hybridomas suggests that in CTL distinct signals stimulate killing activity and IL2 production. The results also suggest that IFN-cdB and IFN-y trigger the cytolytic program through an alternative activation pathway which does not involve the T cell receptor.