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Systematic Multivariate Optimization of Separation of Auxins by Capillary Electrophoresis Based on Overlapping Resolution Mapping Scheme

✍ Scribed by J. Liu; S. F. Y. Li

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 229 KB
Volume: 11
Category: Article
ISSN: 0269-3879
DOI: 10.1002/(sici)1099-0801(199701)11:1<1::aid-bmc606>3.0.co;2-#

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✦ Synopsis

In this paper, the overlapping resolution mapping (ORM) scheme was applied in the separation of 15 plant auxins by capillary electrophoresis. An interpretative optimization approach was utilised to predict the optimum buffer pH, and the concentrations of cu-, /3-, and ycyclodextrins. A four-dimensional overlapping resolution mapping scheme was developed to optimize these four parameters simultaneously. By conducting sixteen prelanned experiments, global optimum conditions for the separation within an acceptable analysis time could be obtained.

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