Systematic investigation of enantiomer resolution ofN-pivaloyl derivatized amino acid esters. Preparation of glass capillary columns coated with the chiral phase XE-60-L-val-pea
✍ Scribed by Aichholz, R. ;Fischer, P.
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 472 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0935-6304
No coin nor oath required. For personal study only.
✦ Synopsis
A laboratory working procedure is described for silanization of glass capillaries with bis(cyanopropyl)tetramethyldisiloxane, prior to coating with XE-60-~-valine-(S)-a-phenylethylamide as chiral stationary phase. GC columns thus prepared exhibit good inertness and no long-term deterioriation of enantioselectivity up to 220'C.A general procedure is given for derivatization of simple and of polyfunctional amino acids, in a batch mixture, simultaneously, and completely at both the N-and the C-terminus and all other protic functions. Within the seven series of N-pivaloyl amino acid esters tested (methyl to neopentyl), baseline separation is obtained for all R/S pairs, including the proline derivative.