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Systematic evaluation of FRAP experiments performed in a confocal laser scanning microscope

โœ Scribed by S. SEIFFERT; W. OPPERMANN

Book ID
108864109
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
380 KB
Volume
220
Category
Article
ISSN
0022-2720

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## Abstract We describe a method to obtain the brightness and number of molecules at each pixel of an image stack obtained with a laser scanning microscope. The method is based on intensity fluctuations due to the diffusion of molecules in a pixel. For a detector operating in the analog mode, the v