𝔖 Bobbio Scriptorium
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Systematic evaluation of avidin–biotin interaction by fluorescence spectrophotometry

✍ Scribed by Shuguo Sun; Xi Huang; Meihu Ma; Ning Qiu; Zhaoxia Cai; Zhang Luo; Nichia Primadani Alies


Book ID
113880808
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
377 KB
Volume
89
Category
Article
ISSN
1386-1425

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The binding of avidin (Avi) with biotin (Bio) and some Bio analogues (biotin methyl ester, desthiobiotin, 2ºiminobiotin and diaminobiotin) was studied by means of Fourier transform Raman spectroscopy. The di †erence in affinity between the protein and these ligands provides a detailed picture of the