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Systematic comparison of the fidelity of aRNA, mRNA and T-RNA on gene expression profiling using cDNA microarray

โœ Scribed by Yao Li; Tao Li; Sanzhen Liu; Minyan Qiu; Zhiyong Han; Zhenling Jiang; Rongyu Li; Kang Ying; Yi Xie; Yumin Mao


Book ID
118470974
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
316 KB
Volume
107
Category
Article
ISSN
0168-1656

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Bacterial messenger RNA (mRNA) is not coherently polyadenylated, whereas mRNA of Eukarya can be separated from stable RNAs by virtue of polyadenylated 3-termini. We have developed a method to isolate Escherichia coli mRNA by polyadenylating it in crude cell extracts with E. coli poly(A) polymerase I