✦ LIBER ✦

Systematic and statistical errors in quantitative evaluation in TLC and HPTLC. 4. Linear calibration

✍ Scribed by Ebel, S. ;Geitz, E. ;Glaser, E.

Publisher: John Wiley and Sons
Year: 1981
Tongue: English
Weight: 377 KB
Volume: 4
Category: Article
ISSN: 0935-6304
DOI: 10.1002/jhrc.1240041006

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✦ Synopsis

Abstract

Analytical results are often obtained from reflectance or fluorescence measurements in TLC or HPTLC with the aid of calibration lines. Curve fitting may not be done by conventional least squares methods for both the independent and the dependent variables are erroneous; errors occur in the volumes spotted on to the plate and in the reflectance/fluorescence measurements. Therefore the sum of the squares of the distances to the calibration line has to be minimized instead of the squared sum of the errors of the dependent variable. The algorithms used are derived and explained. Implications of error propagation for the analytical result are also given.

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