System Issues: Organ variation in the mutagenicity of ethylnitrosourea in Muta™ Mouse: Results of the collaborative study on the transgenic mutation assay by JEMS/MMS
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 918 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
A collaborative study of the transgenic mouse mutation assay was performed by a subgroup of the Mammalian Mutagenesis Study Group (MMS), which is a suborganization of the Environmental Mutagen Society of Japan (JEMS). Twenty-six laboratories participated in this collaboration, and ethylnitrosourea (ENU) mutagenesis was studied in eight organs of /acZ transgenic mice (MutaTM Mouse)liver, spleen, bone marrow, brain, lung, kidney, urinary bladder, and heart. Mice were treated by a single intraperitoneal (ip) injection of 150 mg/kg ENU, and the lacZ mutant frequency (MF) was analyzed by positive selection after 3and 14day expression times. The MF in the control group was ~~ ~ similar for all organs, approximately 40-60 x ENU increased MF in all organs except the brain with the highest values (more than 10 times the control value) observed in bone marrow on days 3 and 14, and in spleen on day 14. The MF in urinary bladder increased over 400 x and MFs in liver and lung were more than 150 x 1 0-6 on day 14, although no increases were apparent in these organs on day 3. Approximately a doubling of control values was observed in kidney and heart but these were less than 100 x 1 O-6. These results demonstrated that ENU induces orgonspecific mutagenesis with specific expression periods.