System for acquisition and real-time processing of multidimensional slit-scan flow cytometric data

✍ Scribed by R. D. Robinson; D. M. Wheeless; S. J. Hespelt; L. L. Wheeless

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 683 KB
• Volume: 11
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/cyto.990110308

✦ Synopsis

The high-speed sampling requirements of multidimensional slit-scan signals (cell contours) have typically required custom hardware. This specialized hardware has often lacked the flexibility to adapt to varying instrument setups and experimental requirements. A hardware and software system capable of sampling multiple slit-scan cell contours at rates of up to 40 MHz with 10-bit resolution is described. It utilizes commercially available CAMAC transient recorders, a Digital Equipment Corp. PDP-11/83 computer, and custom hardware for signal conditioning and trigger generation. The modular design of the software system allows various hardware options with minimal additional coding. Real-time digital processing checks each cell contour for multiple peaks; extracts morphological features such as width, height, and area; accumulates gated histograms of these data; and optionally saves the derived data, selected contours, or both into list mode files on disk.