The transesterification method used by MAHADEVAN and LUNDBERG for cholesterol esters synthesis, following the scheme cholesterol acetate + fatty acid methyl ester + cholesterol ester + methyl acetate, has been adapted to the microscale (1 to 10 pM) imposed by the use of labelled molecules of high specific activity.
This method is particularly suitable for the synthesis of polyethylenic fatty acid cholesterol esters : it has unable us to obtain the following esters : 414C and 7a3H cholesteryl linolenate and arachidonate, cholesteryl linolenate U14C and arachidonate (5-6, 8-9, 11-12, 14-15)3H; the mixture of these two types of esters of high specific activity give rise to series of doubly labelled esters, at the user's convenience.
RESUME
La methode de transesterification utilisee par MAHADEVAN et LUNDBERG pour synthetiser des esters du cholestCrol selon : acCtate de cholestCryle + ester mtthylique d'acides gras -> ester du cholesterol + acCtate de mtthyle, a Ct C adaptee aux microquantitks (1 a 10 pM) de 1'Cchelle imposte par l'emploi de molCcules marquees d'activitks specifique tlevCe.
Cette methode particulikrement adaptte aux synthbes d'esters d'acides gras polyethyltniques a permis d'obtenir les esters suivants : IinolCnate et arachidonate de cholestkryle 414C ou 7a3H, linolenate U1*C de cholesteryle et arachidonate (5-6, 8-9, 11-12, 14-15)3H de cholestkryle : le mklange de ces * R e p le 29 d h m b r e 1964. Actuellement Attach6 de Recherches a 1'1. N. S. E. R. M Les details du mode operatoire ont ett donnes dans une publication anterieure [18].