<p><span>This volume presents state-of-the art methods for the synthesis, design, assembly, post synthesis processing, and application of synthetic DNA to modern biotechnology. Chapters are divided into three general sections focusing on protocols for the computational design of synthetic DNA sequen
Synthetic DNA: Methods and Protocols
โ Scribed by Randall A. Hughes
- Publisher
- Humana Press
- Year
- 2016
- Tongue
- English
- Leaves
- 263
- Series
- Methods in Molecular Biology 1472
- Category
- Library
No coin nor oath required. For personal study only.
โฆ Synopsis
This volume presents state-of-the art methods for the synthesis, design, assembly, post synthesis processing, and application of synthetic DNA to modern biotechnology.ย Chapters are divided into three general sections focusing on protocols for the computational design of synthetic DNA sequences, the synthesis, assembly and cloning of synthetic DNA, and post-synthesis error reduction strategies.ย Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
ย
Authoritative and cutting-edge, Synthetic DNA: Methods and Protocols aims to help researchers further their research on manipulate DNA sequences.
โฆ Table of Contents
Front Matter....Pages i-xiii
Front Matter....Pages 1-1
Front Matter....Pages 3-11
Front Matter....Pages 13-34
Back Matter....Pages 35-45
....Pages 47-47
โฆ Subjects
Human Genetics
๐ SIMILAR VOLUMES
<p>This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic
<p><p>The rapid expansion of synthetic biology is due to the design and construction of synthetic gene networks that have opened many new avenues in fundamental and applied research. <i>Synthetic Gene Networks: Methods and Protocols</i> provides the necessary information to design and construct synt
<p><p>This volume outlines key steps associated with the design, building, and testing of synthetic metabolic pathways<i></i>for optimal cell factory performance and robustness, and illustrates how data-driven learning from these steps can be used for rational cost-effective engineering of cell fact
This book assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of <i>cis</i>-regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bio