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Synthesis/Degradation Ratio Mass Spectrometry for Measuring Relative Dynamic Protein Turnover

โœ Scribed by Cargile, Benjamin J.; Bundy, Jonathan L.; Grunden, Amy M.; Stephenson, James L.


Book ID
121409309
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
184 KB
Volume
76
Category
Article
ISSN
0003-2700

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A method was developed for measuring protein fractional synthetic rates using the N-methoxycarbonylmethyl ester (MCM) derivative of L-[ 1-13C ] valine and on-line gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). The derivatization procedure can be performed rapidly and GC s