Pyridine derivative 2-hydrazino-6-methyl-4-(methoxymethyl)-5-nitropyridine-3-carbonitrile (2) has been obtained from 2-chloro-4-(methoxymethyl)-6-methyl-5-nitropyridine-3-carbonitrile (1) by novel protocol and its structure was determined by X-ray diffraction method. An analysis revealed some structural differences in 2 compared to corresponding 3-cyano-5-nitro-pyridines and 2-hydrazinopyridines. Supramolecular structure contains one NAHÁ Á ÁN and two NAHÁ Á ÁO hydrogen bonds, as well as one CAHÁ Á ÁO hydrogen bond. Structural features have been also studied by FT-IR, FT-R and NMR spectroscopy. FT-IR dilution experiments showed that the character of intermolecular NHÁ Á ÁO and NHÁ Á ÁN interactions in solution depends on concentration. The spectral properties were investigated by UV-vis absorption and fluorescence spectroscopy techniques. Absorption spectra of compounds have been recorded in protic and aprotic solvents in the range 200-550 nm and solvent effects on the absorption spectra of this compound are interpreted. Absorption spectra of aqueous solution of compound 2 were also recorded at different temperatures and pH values and protonation constant was calculated. Unlike previously studied structurally similar compound 1, the title compound 2 does not emit fluorescence.