Synthesis of β-amyloid precursor peptide and presenilin segments

✍ Scribed by Lajos Baláspiri; Ülo Langel

• Book ID: 105360071
• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 56 KB
• Volume: 7
• Category: Article
• ISSN: 1075-2617
• DOI: 10.1002/psc.305

✦ Synopsis

Abstract

It seems likely that the β‐amyloid precursor protein (APP) and the presenilins (PS‐1/2) play important roles in the development of Alzheimer's disease (AD). Attempts to mimic the biochemical actions of these proteins are often made by the application of fragments of these proteins. However, the synthesis of these segments by conventional methods of peptide synthesis is problematic. We have synthesized several C‐terminal fragments of APP and PS‐1/2 by solid‐phase synthesis through combination of automatic and manual methods of synthesis. This permits solution of the ‘difficult sequences’ in the solid‐phase synthesis of these peptides. Some details of the syntheses of nine segments are presented in this paper. Copyright © 2001 European Peptide Society and John Wiley & Sons, Ltd.