Synthesis of two series of cysteine-specific spin labels containing a 15N substituted nitroxide

✍ Scribed by Ge Wu

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: French
• Weight: 394 KB
• Volume: 36
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580361207

✦ Synopsis

Abstract

Synthesis of two series of spin labels with a cysteine‐reactive group, either maleimide or methanethiosulfonate, and a ^15^N substituted nitroxide separated at variable distance is reported. In the synthesis, the cysteine‐reactive groups were first incorporated into the target molecule and the ^15^N substituted nitroxide was introduced in the last step via a mild reaction without destroying the cysteine‐reactive groups. The synthetic routes described here are applicable to the synthesis of spin labels with either ^15^N substituted or “normal” ^14^N nitroxide. Spin labeling of cysteine residues in rhodopsin by the newly synthesized spin labels was also demonstrated.