Synthesis of tritium labelled cholecystokinin derivative : [3H]-Boc-[Nle28, 31]- CCK27–33
✍ Scribed by N. A. Sasaki; S. Funakoshi; P. Potier; J.-L. Morgat; R. Genet; G. Gacel; B. Charpentier; B. P. Roques
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- French
- Weight
- 456 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0022-2135
No coin nor oath required. For personal study only.
✦ Synopsis
The s y n t h e s i s o f a new a c e t y l e n i c a n a l o g u e o f t h e C-t e r m i n a l hep t ap ep t i de segment o f c h o l e c y s t o k i n i n ( C C K ) i n which t h e Met28 and Met31 r e s i d u e s a r e r e p l a c e d by two a c e t y l e n i c p r e c u r s o r s o f n o r l e u c i n e ( N l e ) , L -2amino-4-hexynoic a c i d ( A h a ) , i s d e s c r i b e d . R e d u c t i v e t r i t i a t i o n o f t h i s a c e t y l e n i c hep t apep t i d e Bo c -[ Aha l e d t o t h e l a b e l l e d 13H) -Boc-(Nle28 31] -CCK CCK27, 33 which d i s p l a y s a s p e c i f i c a c t i v i t y o f a b o u t 150 Ci/mnol. According t o i t s f u l l b i o l o g i c a l p o t e n c y , t h i s CCK a n a l o g u e can be u s e d f o r v a r i o u s b i o l o g i c a l a s s a y s i n c l u d i n g b i n d i n g s t u d i e s . KEY WORDS : p e p t i d e s y n t h e s i s , tritium l a b e l l i n g , c h o l ec y s t o k i n i n h e p t a p e p t i d e a n a l o g u e , CCK r e c e p t o r , L-2-amino-&h e x y n o i c a c i d .