Synthesis of the self-complementary RNA-DNA hybrid:R(GCG)D(TATACGC)

✍ Scribed by C. A. A. van Boeckel; G. A. van der Marel; G. Wille; H. Westerink; A. H. J. Wang; J. R. Mellema; C. Altona; J. H. van Boom

• Book ID: 104588372
• Publisher: Elsevier Science
• Year: 2010
• Tongue: English
• Weight: 143 KB
• Volume: 100
• Category: Article
• ISSN: 0165-0513
• DOI: 10.1002/recl.19811001011

✦ Synopsis

Abstract

A nucleic acid fragment containing a RNA trimer and a DNA heptamer wich are linked by a 3′‐5′‐internucleotide phosphodiester bond has been synthesized by a phosphotriester approach. The sequence of the nucleosides is such that they will form a self‐complementary double‐helical fragment which may serve as a probe to get more insight into the process of discontinuous DNA replication.