In order to investigate the catalytic activity for the hydrolysis of phosphodiester and the cleavage of nucleic acids, ribose-containing polymers 11, 12 and 13 were synthesized. While polymer 11 showed no catalytic activity, polymers 12 and 13 catalyzed the cleavage of nucleic acids and the hydrolys
Synthesis of the polysaccharide, (1→5)-α-D-ribofuranan and its catalytic activities for the hydrolysis of phosphates and the cleavage of nucleic acids
✍ Scribed by Man Jung Han; Kyung Soo Yoo; Young Heui Kim; Hong Youb Kim; Hyun Joon Shin; Ji Young Chang
- Publisher
- The Polymer Society of Korea
- Year
- 2004
- Tongue
- English
- Weight
- 400 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1598-5032
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## Abstract D‐Glyceraldehyde 3‐phosphate (=D‐GAP; **2**) was prepared by an improved chemical method (__Scheme 2__), and it was then employed to synthesize 1‐deoxy‐D‐xylulose 5‐phosphate (=DXP; **3**) which is enzymatically one of the key intermediates in the MEP (**4**) terpenoid biosynthetic path
Improved methods for the activation and assay of D-ribulose-1,5-bisphosphate carboxylase and oxygenase are described. The importance of fully activating the enzyme before starting either reaction is emphasized. The enzyme is activated by preincubation with 20 mM M&l, and 10 mM NaHCO,, pH 8.6. To avo