Synthesis of the minor fetal hemoglobin Fic in colonies of erythropoietic precursors isolated from human umbilical cord blood
✍ Scribed by E. C. Abraham; A. L. Reese; M. Stallings; A. Abraham; G. J. Garbutt; T. H. J. Huisman
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 385 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0361-8609
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✦ Synopsis
The biosynthesis of the minor hemoglobin F,c, which contains acetylated y chains, and the major hemoglobin F, was studied during erythroid cell differentiation and maturation in cultures of erythroid precursors isolated from five human umbilical cord blood samples. A gradual decrease in the synthesis of Hb F,, was observed during the maturation of the erythroid cells when cultured from 7 to 15 days. The synthesis of Hb F, did not show a consistent change; however, the relative synthesis of Hb F,, was the highest in the 7-day-old colonies and decreased at day 15 to levels observed in the reticulocytes. The addition of sodium butyrate, which is known to promote histone acetylation, significantly increased the synthesis of Hb F,, in 7-day-old colonies.