✦ LIBER ✦
Synthesis of the herpes simplex virus type 1trans-activator Vmw65 in insect cells using a baculovirus vector
✍ Scribed by John P. Capone; Geoffrey Werstuck
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 518 KB
- Volume
- 94
- Category
- Article
- ISSN
- 0300-8177
No coin nor oath required. For personal study only.
✦ Synopsis
Vmw65, the Herpes Simplex Virus trans-activator of immediate-early genes, was expressed in insect cells using a recombinant baculovirus expression vector and partially purified. Insect cell-derived Vmw65 was shown to be indistinguishable from authentic Vmw65 present in purified HSV-1 virions based on electrophoretic mobility, immunoreactivity with a monoclonal antibody, and ability to interact with cellular factors to form a protein/DNA complex with oligonucleotides containing a TAATGARAT element.