Synthesis of the fatty sterol bound protein for a new sterol antibody

✍ Scribed by Byung Ju Kim; Satoshi Yamada; Tadashi Funada; Yoshihito Kadoma; Hiroyuki Morita

• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 109 KB
• Volume: 10
• Category: Article
• ISSN: 0960-894X
• DOI: 10.1016/s0960-894x(99)00694-0

✦ Synopsis

AbstractÐFor the purpose of applying the particular antibodies as a new diagnostic procedure for atherosclerosis and related diseases, we successfully achieved the synthesis of the fatty sterol with a linker, then linked the target protein to this sterol. Synthesis was started from pregnenolone and achieved by the Grignard reaction with pentenyl magnesium bromide, regioselective photoaddition of thiolacetic acid toward the 25-double bond, esteri®cation of 3-OH with linoleic anhydride, in situ conjunction of the cross-linker (MBS) to the thiol group after selective deprotection from its acetyl ester, and ®nally by the reaction with protein such as KLH or albumin through this linker.