✦ LIBER ✦
Synthesis of site-specifically deuterated arachidonic acid derivatives containing a remote tritium radiolabel
✍ Scribed by Chris M. McGinley; Wilfred A. van der Donk
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- French
- Weight
- 168 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
The synthesis of arachidonic acid derivatives containing site-specifically incorporated deuterium atoms and also a remote tritium label are described. Deuterium incorporation at the C11 and/or C15 position was achieved using Wittig chemistry, while the radiolabel was introduced at a remote position using [(3)H]NaBH(4) as the radiolabel source. These compounds can be used to measure secondary kinetic isotope effects for both cyclooxygenase and lipoxygenase enzymes under aerobic turnover with high precision.