Synthesis of Silver Nanoparticle–Hollow Titanium Phosphate Sphere Hybrid as a Label for Ultrasensitive Electrochemical Detection of Human Interleukin-6
✍ Scribed by Juan Peng; Li-Na Feng; Zhong-Jie Ren; Li-Ping Jiang; Jun-Jie Zhu
- Book ID
- 104593878
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 801 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1613-6810
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✦ Synopsis
Abstract
A silver nanoparticle–hollow titanium phosphate sphere (AgNP–TiP) hybrid is successfully synthesized and used as a label for electrochemical detection of human interleukin‐6 (IL‐6). Hollow TiP spheres with a diameter of 430 nm and an average thickness of 40 nm are synthesized by a template approach. The AgNPs are incorporated in situ into the TiP shell via an exchange process. The as‐prepared AgNP–TiP hybrid shows outstanding biocompatibility, good dispersity and solubility in water, and high silver loading properties (289.2 mg of silver in 1.0 g of TiP). These advantages make the AgNP–TiP hybrid an effective candidate as an amplification label in immunoassay systems. Herein, the as‐prepared AgNP–TiP hybrid is attached to a signal antibody (Ab~2~) to produce Ab~2~–AgNP–TiP labels in the fabrication of an electrochemical immunosensor. The nanoparticle‐based amplification labels, upon coupling with a magnetic sensing array, give rise to an extremely sensitive response to IL‐6 in a linear range of 0.0005–10 ng mL^−1^ with a detection limit of 0.1 pg mL^−1^. The proposed sensor exhibits high specificity, good reproducibility, and long‐term stability, and may be a promising technique for protein and DNA detection.