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Synthesis of selectively radiolabeled hexasaccharides for the determination of enzymatic regioselectivity

✍ Scribed by J. C. McAuliffe; M. Ujita; M. Fukuda; O. Hindsgaul

Book ID
110248999
Publisher
Springer US
Year
1999
Tongue
English
Weight
113 KB
Volume
16
Category
Article
ISSN
1573-4986

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A convenient method for enzymatic synthe
A convenient method for enzymatic synthesis of radiolabelled glucose-1,6-bisphosphate
✍ Tom Hamborg Nielsen; Bente Wischmann; Birger Lindberg MΓΈller πŸ“‚ Article πŸ“… 1995 πŸ› John Wiley and Sons 🌐 French βš– 309 KB

## Abstract A convenient method to synthesize radiolabelled glucose‐1,6‐bisphosphate (Glc‐1,6‐P~2~) of high specific activity and in a high yield is reported. The method is based on enzymatic formation of glucose‐6‐P from glucose and ATP using hexokinase. The labelled glucose‐6‐P is then converted