Synthesis of ribosomal protein during the cell cycle of Escherichia coli B/r
β Scribed by Zaritsky, Arieh ;von Meyenburg, Kaspar
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 686 KB
- Volume
- 129
- Category
- Article
- ISSN
- 0026-8925
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The replication of F' lac was studied in exponentially growing cultures of E. coli B/r. The cells were pulse induced for the synthesis of beta-galactosidase and their DNA pulse labelled with 3H thymidine. The cells were then separated into age classes by centrifugation through a sucrose gradient in
The exchange of ribosomal proteins among ribosomes of E. coli has been measured, using a density label technique. As expected most of the proteins do not exchange appreciably. However a substantial fraction of each of proteins S1, S2, S21, L7/L12, L9, L10, L11, L26 and L33 is found to exchange, but