Synthesis of prostaglandins I2 and E2 by the canine gallbladder in vitro: Studies using a new incubation chamber

✍ Scribed by D. W. England; Dr B. M. Jaffe; H. Webb; E. L. Hoover

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 635 KB
• Volume: 77
• Category: Article
• ISSN: 0007-1323
• DOI: 10.1002/bjs.1800770218

✦ Synopsis

Using a specially designed incubation chamber, differential synthesis and the response to added arachidonic acid ( 2 5 m ~) and L-U- phosphatidylcholine (LAP; 2 mM) was quantified in gallbladders from male and female dogs. Prostaglandin I , (PGI,) was the predominant prostanoid synthesized, and tissues from females produced much more PGI, under basal conditions than did gallbladders harvested from male dogs. Addition of arachidonic acid stimulated PGI, synthesis by almost looper cent. Arachidonate-stimulated mucosal and serosal production of PGI, were (mean(s.e.m.)) comparable, 343(178) and 375( 89)pg/cm2/min, respectively. Gallbladders from female animals synthesized significantly more PGI, than did tissue from males. Indomethacin inhibited PGI, synthesis in a dose-response manner; at 7 x M , prostanoid synthesis was inhibited by >80 per cent. Arachidonic acid did not stimulate prostaglandin E , (PGE,) production by gallbladder tissue. L A P similarly stimulated PGI, biosynthesis, but in contrast to the effect of arachidonic acid, the effect was significantly greater in the serosa than the mucosa, 355(107) and 213( 59)pg/cm2/min, respectively. LAP also stimulated PGE, biosynthesis by the canine gallbladder in a pattern very similar to that of PGI,. Based on the differences in response to the two agents added, we conclude that arachidonic acid and L-cr-phosphatidylcholine stimulate prostaglandin biosynthesis via independent pathways. We advocate the use of the incubation chamber for the assessment of prostanoid biosynthesis by the gallbladder in vitro.