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Synthesis of poly(L-Tyr-L-Glu-L-Ala-Gly) and poly(L-Glu-L-Lys-L-Ala) and their circular dichroism spectra in aqueous solution

✍ Scribed by Allen R. Zeiger; Chang-Hai Lai; Paul H. Maurer

Publisher
Wiley (John Wiley & Sons)
Year
1975
Tongue
English
Weight
779 KB
Volume
14
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

Two sequential polypeptides, poly(O‐benzyl‐L‐Tyr‐γ‐benzyl‐L‐Glu‐L‐Ala‐Gly) and poly(ε‐benzyloxycarbonyl‐L‐Lys‐L‐Glu‐L‐Ala), were synthesized, the former by the pentachlorophenyl ester of the tetrapeptide monomer and the latter by the azide of the tripeptide monomer. After deprotection and dialysis, poly(L‐Tyr‐L‐Glu‐L‐Ala‐Gly) was obtained in 71% yield and had a molecular weight of 53,000. The circular dichroism spectra (CD) of the polymer at pH's 7.2, 10.5, and 11.8 and of oligomers and of the monomer at pH 7.2 indicated that the polymer exists in an α‐helical conformation.

After deprotection, poly(L‐Lys‐L‐Glu‐L‐Ala) was obtained in 37% yield and had a molecular weight of 3000. The CD spectra of the polymer at pH 7.2 and 2.8, and of the monomer at pH 7.2, indicated that the polymer is in a randomly coiled configuration.

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