A novel synthetic route to heterobifunctional poly(ethylene glycol) containing both amino and carboxylate terminal groups was established via anionic polymerization of ethylene oxide using (cyanomethyl)potassium as the initiator, followed by the successive conversion of the end-hydroxyl group to a p
✦ LIBER ✦
Synthesis of poly(ethylene glycol)-bound NADP by selective modification at the 6-amino group of NADP
✍ Scribed by Keiko OKUDA; Itaru URABE; Hirosuke OKADA
- Book ID
- 115123158
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 599 KB
- Volume
- 151
- Category
- Article
- ISSN
- 1432-1327
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## Abstract Covalent modification of purified __Trigonopsis variabilis__ D‐amino acid oxidase using maleimide‐activated poly(ethylene glycol) 5000 yielded a stable bioconjugate in which three surface‐exposed cysteine side chains were selectively derivatized. Compared with the native enzyme, the PEG